Inhibition of Cellulase Activity Fusarium oxysporum f. sp. lycopersici Pathogens in Tomato Plants Using Essential Oils from Citronella in Vitro
DOI:
https://doi.org/10.37637/ab.v8i1.1883Keywords:
essential oils, fusarium oxysporum cellulase, inhibition, tomato plantsAbstract
The purpose of this study is to determine the ability of essential oils from citronella plants to inhibit cellulase enzyme activity in the fungus Fusarium oxysporum f. sp. lycopersici. and evaluate its potential as a disease-control material in tomato plants. This research was carried out in August 2023 until completed in February 2024. The method used in this study is experimental which consists of the process of exploring the fungus that causes disease in tomato plants, microscopic characterization of fungal isolates, molecular identification, inhibition test of fungal colony growth using essential oils from citronella plants, cellulase enzyme activity test, and cellulase enzyme activity inhibition test using essential oils from citronella plants. The variables observed in this study consisted of inhibiting the growth of Fusarium oxysporum f. sp. lycopersici. By essential oils, test the activity of quantitative cellulase enzymes, and cellulase enzymes activity inhibition test using essential oil from citronella plant. The results of microscopic characterization and molecular tests show that the fungal isolate used in this study has 99% similarity with Fusarium oxysporum f. sp. lycopersici. Essential oils extracted from citronella plants effectively inhibit the formation of fungal colonies. This is evidenced by the results of observation at the age of 7 HSI mushrooms with the growth diameter of fungal colonies of 5.06 cm, 3.56 cm, and 2.44 cm after applying essential oils with concentrations of 333 ppm, 666 ppm, and 1000 ppm. Results of observation and calculation of cellulase enzyme activity values in Fusarium oxysporum f. sp. lycopersici isolate. Cellulase enzyme activity of 0.831 U/mL was obtained. The application of essential oils with sequential concentrations (333 ppm, 666 ppm, and 1000 ppm) was able to decrease the activity of cellulase enzymes, with activity inhibition of 31%, 39%, and 44% in Fusarium oxysporum f. sp. lycopersici isolates.
References
Andi, M., Darma, T., & Rahmawati, T. (2022). Identification of Growth Hormone-Producing Rhizosphere Microbes on Rice Plant (Oryza sativa L.). Agro Bali. 5(3): 608-615. https://doi.org/10.37637/ab.v5i3.1040
Alma, M.H., Ertas M., Nitz S., Kollmannsberger H. 2007. Chemical composition and content of essential oil from the bud of cultivated Turkish clove. BioResources. 2(2): 265-269. https://cir.nii.ac.jp/crid/1360857595273276032
Central Bureau of Statistics of Indonesia. (2024). Vegetable Crop Production. Retrieved February 11, 2025, from https://www.bps.go.id/id/statistics-table/2/NjEjMg==/produksi-tanaman-sayuran.html
Chui, H., Zhang, C., Li, C., & Lin, L. (2018). Antimicrobial mechanism of clove oil on listeria monocytogenes. Food Control. 9(4): 150-146. https://doi.org/10.1016/j.foodcont.2018.07.007
El Modafar, C., Tantaoui, A., & El Boustani, E. (2000). Effet de L'acide Caféoylshikimique des Racines du Palmier Dattier sur L'activité et la Production des Enzymes Hydrolytiques de Fusarium oxysporum f. sp. albedinis. Journal of Phytopathology. 148(2): 101-108. https://doi.org/10.1046/j.1439-0434.2000.00472.x
Elfina, Y., Ali, M., & Aryanti, L. (2015). Test of several concentrations of forest betel leaf flour extract (Piper aduncum L.) to control anthracnose disease in post-harvest red chili. SAGU, 14(2), 18–27. https://sagu.ejournal.unri.ac.id/index.php/JSG/article/view/3006
Freeman, S., & Maymon, M. (2000). Reliable Detection of the Fungal Pathogen Fusarium oxysporum f.sp. albedinis, Causal Agent of Bayoud Disease of Date Palm, Using Molecular Techniques. In Phytoparasitica 28(4). http://www.phytoparasitica.org
Hamid, Abdullah B., & Slamet P. 2015. Selection of Aspergillus niger and Trichoderma reesei. Strains to Obtain High Cellulase Enzyme Activity In Solid Fermentation Using Bagas. XIV’s RAPI National Symposium. Faculty of Engineering, Diponegoro University Semarang. ISSN 1412-9612. http://hdl.handle.net/11617/6604
Hani S, Herry N, Wiyatiningsih S. (2024). Compatibility of Awar-awar (Ficus septical) Leaf Extract and Citronella Extract (Cimbopogon nordus L.) in Inhibiting Collectrotrichum capsici. Fungus. Agro Bali. 7(1): 212-217. https://doi.org/10.37637/ab.v7il.1402
IBPE Putra, IN Wirjana, AAIAM Laksmiwati. 2019. Cellulase and Amylase from Rotten Palm Leaves (Borassus flabelliformis) and Inhibition Test with Lemongrass and Clove Oils. Cakra Kimia (Indonesian E-Journal of Applied Chemistry): 7(2), 140-148. https://ojs.unud.ac.id/index.php/cakra/article/download/56190/33153
Istikomah, N., N. H. Alami., & K. I. Purwani. (2015). Effect of Pamelo Orange Peel Extract on Fusarium oxysporum Fungal Infection in Tomato Plants. SCIENCE and ART, 4(2): 2337-3520. https://dx.doi.org/10.12962/j23373520.v4i2,13460
Jumepaeng, T., Prachakool, S., Luthria, D. L., & Chanthai, S. (2013). Determination of antioxidant capacity and α-amylase inhibitory activity of the essential oils from citronella grass and lemongrass. In International Food Research Journal (Vol. 20, Number 1). https://www.proquest.com/scholarly-journals/determination-antioxidant-capacity-alpha-amylase/docview/1426008445/se-2
Mahalwal, V. S., & Ali, M. (2003). Volatile constituents of Cymbopogon nardus (Linn.) Rendle. In Flavour and Fragrance Journal (Vol. 18, Number 1, pp. 73–76). https://doi.org/10.1002/ffj.1144
Manici, L. M., Caputo, F., & Saccà, M. L. (2017). Secondary metabolites released into the rhizosphere by Fusarium oxysporum and Fusarium spp. as underestimated component of nonspecific replant disease. Plant and Soil, 415(1–2), 85–98. https://doi.org/10.1007/s11104-016-3152-2
Marei, G. I. K., & Abdelgaleil, S. A. M. (2018). Antifungal potential and biochemical effects of monoterpenes and phenylpropenes on plant pathogenic fungi. Plant Protection Science, 54(1), 9–16. https://doi.org/10.17221/9/2017-PPS
Maria Otang, Yoseph Yakob, Da Rato, Sarlina Noni. (2021). Consumer Preferences for Purchasing Watermelon (Citrullus vulgaris) in the Practical Garden of the Faculty of Agriculture, Nusa Nipa University, Indonesia. Scientific Journal of Wahana Pendidikan. 7(2), 441-455. https://jurnal.peneliti.net/index.php/JIWP
Mrudula, S., & Murugammal, R. (2011). Production Of Cellulase By Aspergillus Niger Under Submerged And Solid State Fermentation Using Coir Waste As A Substrate. Brazilian Journal of Microbiology, 42, 1119–1127. https://doi.org/10.1590/S1517-83822011000300033
Rasul, F., Afroz, A., Rashid, U., Mehmood, S., Zeesha, N. (2015). Screening and Characterization of Cellulas Producing Bacteria from Soil and Waste (Molasse) of Sugar Industry. In j Biosci. 6(3): 230-238. https://dx.doi.org/10.1262/ijb/6.3.230-5
Sinaga, S.M. (2006). Isolation and Selection of Endophytic Bacteria for Control of Blood Disease in Banana Plants. J. Tropical HPT. 11(2), 113-121. https://doi.org/10.23960/j.http.211113-121
Suprapta, D. N., IB.G. Darmayasa, N. Arya, I G. R.M. Temaja and K. Ohsawa. 2003. Bacterial Activity of Spaeranthus indices Extract Against Ralstonia solanacearum in Tomato. Journal of ISSAAS. 9: 69-74. https://issaasphil.org/wp-content/uploads/2019/06/journal-ISSAAS-vol-25-no-1June-2019.pdf
Vinola J., and Thiruneelakandan G. 2015. Enzymatic Activity of Marine Lactobacillus Species from South East Coast of India. IJISET. 2(1), 2348 – 7968. http://www.ijiset.com/
Yanti, R., Nurdiawati, H., Cahyanto, M. N., & Pranoto, Y. (2020). Component Identification and Antifungal Potential Test of Kitchen Lemongrass Essential Oil (Cympobogon citratus) Against Aflatoxin-Producing Fungi. AGRITEKNO: Journal of Agricultural Technology, 9(2), 72–80. https://doi.org/10.30598/jagritekno.2020.9.2.72.
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