Growth Inhibition of Botrytis cinerea Fungus on Strawberry (Fragaria sp.) Using Kaffir Lime (Citrus hystrix) Leaf Essential Oil Emulsion

Authors

  • Nita Nofia Fakultas Pertanian - Universitas Brawijaya, Indonesia https://orcid.org/0009-0004-3972-8696
  • Mintarto Martosudiro Fakultas Pertanian - Universitas Brawijaya, Indonesia
  • Anton Muhibuddin Fakultas Pertanian - Universitas Brawijaya, Indonesia

DOI:

https://doi.org/10.37637/ab.v7i1.1623

Keywords:

Botrytis cinerea, Citrus hystrix, essential oil, inhibition, strawberry

Abstract

Post-harvest damage is caused by biological factors such as the attack of Botrytis cinerea pathogenic fungi. causes gray mold rot disease to be a source of problems in strawberry production. This study aims to determine the potential of kaffir lime leaf essential oil emulsion as an antifungal against Botrytis cinerea. The fungus was isolated from strawberry fruit samples that showed symptoms of gray mold disease. Fungal isolation was carried out through the direct planting method and antifungal inhibitory ability test by food poisoned method with potato extract agar media. This experiment used a completely randomized design consisting of 6 treatments with 5 repetitions. The results showed that kaffir lime leaf essential oil emulsion contains secondary metabolites that can inhibit 100% of the growth of Botrytis cinerea pathogenic fungal colonies. Essential oil concentrations of 0.10%, 0.15%, and 0.20% had a significant effect on the growth of Botrytis cinerea pathogenic fungi.

References

Agustina, A., & Jamilah, M. (2021). Kajian Kualitas Minyak Serai Wangi (Cymbopogon winterianus Jowitt.) pada CV AB dan PT. XYZ Jawa Barat. Agro Bali: Agricultural Journal, 4(1), 63–71. https://doi.org/10.37637/ab.v4i1.681

Angraeni, L. (2019). Pengelolaan Penyakit Pasca Panen pada Buah dan Sayur dengan Pemanfaatan Ekstrak Alami. Jurnal Teknologi Pengolahan Pertanian, 1(1), 18. https://doi.org/10.35308/jtpp.v1i1.1404

Anitasari, S. D., & Sari, D. N. R. (2021). The Activities Of Combination Citrus hystrix Peel Extract and Carica papaya Leaves Extract Against Candida albicans and Escherichia coli. Medicra (Journal of Medical Laboratory Science/Technology), 4(1), 17–21. https://doi.org/10.21070/medicra.v4i1.1359

Ardiansyah, & Mulia, A. (2016). Karakteristik dan Mutu Teh Kulit Melinjo. Seminar Hasil Penelitian Dan Pengabdian Masyarakat Dana BOPTN, 1(1), 100–103. https://publikasi.polije.ac.id/index.php/jii/article/view/289

Azmi, A. F., Kamil, I., Fahiratunnisa, N. A., Pramita, J., Maulidia, Y., & Sari, R. (2023). Efektivitas Edible Coating Spray Gel Lidah Buaya (Aloe vera) dan Daun Sirih Effectiveness of Edible Coating Spray Gel Aloe Vera (Aloe vera) and Betel Leaf (Piper betle) as Food Sanitizers. Pharmaceutical Journal of Indonesia, 8(2), 157–163.

Diana, N., & Khotimah, S. (2014). Penghambatan Pertumbuhan Jamur Fusarium oxysporum Schlecht Pada Batang Padi (Oryza sativa L.) Menggunakan Ekstrak Metanol Umbi Bawang Mekah (Eleutherine palmifolia Merr.). Protobiont, 3(2), 225–231.

Elfina, Y., Ali, M., & Aryanti, L. (2015). Uji Beberapa Konsentrasi Ekstrak Tepung Daun Sirih Hutan (Piper aduncum L.) untuk Mengendalikan Penyakit Antraknosa pada Buah Cabai Merah Pasca Panen. Sagu, 14(2), 18–27.

Erhonyota, C., Edo, G. I., & Onoharigho, F. O. (2023). Comparison of poison plate and agar well diffusion method determining the antifungal activity of protein fractions. Acta Ecologica Sinica, 43(4), 684–689. https://doi.org/10.1016/j.chnaes.2022.08.006

Hapsari, E., Soesanto, L., & Mugiastuti, E. (2021). Viabilitas dan Virulensi Tujuh Belas Tahun Penyimpanan Fusarium oxysporum Schlecht. f.sp. zingiberi Trujillo dalam Tanah Steril. Media Pertanian, 6(2), 72–82.

Holz, G., Coertze, S., & Williamson, B. (2007). The ecology of botrytis on plant surfaces. Botrytis: Biology, Pathology and Control, 9–27. https://doi.org/10.1007/978-1-4020-2626-3_2

Li, W. R., Shi, Q. S., Ouyang, Y. S., Chen, Y. Ben, & Duan, S. S. (2013). Antifungal effects of citronella oil against Aspergillus niger ATCC 16404. Applied Microbiology and Biotechnology, 97(16), 7483–7492. https://doi.org/10.1007/s00253-012-4460-y

Mertely, J. C., Oliveira, M. S., & Peres, N. A. (2018). Botrytis Fruit Rot or Gray Mold of Strawberry. Edis, 2018(1), 1–4. https://doi.org/10.32473/edis-pp152-2018

Mirzaei, S., & Goltapeh, E. M. (2014). Taxonomical studies on the genus Botrytis in Iran Taxonomical studies on the genus Botrytis in Iran. Journal of Agricultural Technology, January 2007.

Newell, P. D., Fricker, A. D., Roco, C. A., Chandrangsu, P., & Merkel, S. M. (2013). A Small-Group Activity Introducing the Use and Interpretation of BLAST. Journal of Microbiology & Biology Education, 14(2), 238–243. https://doi.org/10.1128/jmbe.v14i2.637

Nielsen, K., & Protection, C. (2002). PCR Detection and RFLP Differentiation of Botrytis Species facilitate the rapid appraisal of interactions. Plant Disease, 86(6), 682–686.

Nugraheni, A. septianingsih, Djauhari, S., Cholil, A., & Utomo, E. P. (2014). Potensi Minyak Atsiri Serai Wangi (Cymbopogon winterianus) sebagai Fungisida Nabati terhadap Penyakit Antraknosa (Colletotrichum gloeosporioides) pada Buah Apel (Malus sylvestris Mill). Jurnal Hpt, 2(4), 42–50.

Pumival, P., Tadtong, S., Athikomkulchai, S., & Chittasupho, C. (2020). Antifungal Activity and the Chemical and Physical Stability of Microemulsions Containing Citrus hystrix DC Leaf Oil. Natural Product Communications, 15(9), 1–12. https://doi.org/10.1177/1934578X20957755

Santosa, E. P., Firdaus, M., & Novianti, T. (2016). Daya Saing Komoditas Hortikultura Negara Berkembang dan Negara Maju di Pasar Internasional. Jurnal Ekonomi Dan Kebijakan Pembangunan, 5(2), 10–31.

Saputera, M. M. A., Marpaung, T. W. A., & Ayuchecaria, N. (2019). Konsentrasi Hambat Minimum (KHM) Kadar Ekstrak Etanol Batang Bajakah Tampala (Spatholobus littoralis Hassk) terhadap Bakteri Escherichia coli Melalui Metode Sumuran. Jurnal Ilmiah Manuntung, 5(2), 167–173.

Sudania, S., Ropalia, R., & Kusmiadi, R. (2023). The Inhibitory Potential of Botanical Fungicides Against Colletotrichum capsici the Causal Agent of Anthracnose on Chili in-Vitro. CROPSAVER - Journal of Plant Protection, 6(1), 40. https://doi.org/10.24198/cropsaver.v6i1.44219

Warsito, Palungan, M. H., & Utomo, E. P. (2017). Profiling study of the major and minor components of kaffir lime oil (Citrus hystrix DC.) in the fractional distillation process. The Pan African Medical Journal, 8688, 1–9. https://doi.org/10.11604/pamj.2017.27.282.9679

Waryat, W., & Handayani, Y. (2020). Implementasi Jenis Kemasan Untuk Memperpanjang Umur Simpan Sayuran Pakcoy. Jurnal Ilmiah Respati, 11(1), 33–45. https://doi.org/10.52643/jir.v11i1.847

White, T. J., Bruns, T., Lee, S., & Taylor, J. (1990). Amplification and Direct Sequencing of Fungal Ribosomal Rna Genes for Phylogenetics. In PCR Protocols (Issue 1). Academic Press, Inc. https://doi.org/10.1016/b978-0-12-372180-8.50042-1

Wu, Y., OuYang, Q., & Tao, N. (2016). Plasma membrane damage contributes to antifungal activity of citronellal against Penicillium digitatum. Journal of Food Science and Technology, 53(10), 3853–3858. https://doi.org/10.1007/s13197-016-2358-x

Yanti, R., Wulandari, P., Pranoto, Y., & Muhammad Nur Cahyanto. (2017). Karakterisasi, Identifikasi dan Uji Aktivitas Anti Jamur Minyak Atsiri Daun Jeruk Purut (Citrus hystrix) terhadap Aspergillus. Jurnal Teknologi Pertanian, 8(2), 1–7.

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Published

2024-04-30

How to Cite

Nofia, N., Martosudiro, M., & Muhibuddin, A. (2024). Growth Inhibition of Botrytis cinerea Fungus on Strawberry (Fragaria sp.) Using Kaffir Lime (Citrus hystrix) Leaf Essential Oil Emulsion. Agro Bali : Agricultural Journal, 7(1), 118–127. https://doi.org/10.37637/ab.v7i1.1623

Issue

Vol. 7 No. 1 (2024)

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