Characterization of Fusarium oxysporum f. sp. cubense Caused Fusarium Wilt in Donomulyo, Malang Regency, Indonesia
DOI:
https://doi.org/10.37637/ab.v9i2.2749Keywords:
banana plant, fusarium wilt, Foc, PCR amplification, soil-borne diseaseAbstract
Fusarium oxysporum f. sp. cubense (Foc) is a soil-borne fungal pathogen responsible for fusarium wilt, which constitutes one of the major constraints in banana cultivation. Information on the occurrence and molecular characteristics of Foc in Malang Regency, one of the banana-producing areas in East Java, Indonesia, remains limited, hindering effective disease surveillance and management. This study was conducted to isolate and characterize fungal isolates associated with fusarium wilt disease in banana plants using an integrated approach combining morphological characterization, pathogenicity testing, PCR amplification, ITS sequence analysis, and phylogenetic analysis. Three fungal isolates were recovered from symptomatic banana plants collected in Donomulyo, Malang Regency, and one representative isolate (DNM) was selected for molecular characterization based on its morphological similarity to the other isolates. Morphological observations revealed characteristics typical of Fusarium oxysporum. ITS sequence analysis showed 100% query coverage and 100% sequence identity with reference Foc sequences available in GenBank, while phylogenetic analysis clustered the DNM isolate with reference Foc isolates with 94% bootstrap support. Pathogenicity assays demonstrated that the isolate induced typical fusarium wilt symptoms, resulting in an LDI of 16.39% at 14 DAI and an RDI of 41.67% at 45 DAI. These findings provide baseline information on putative Foc isolates in Malang Regency, particularly in the Donomulyo area, and establish an integrated characterization framework to support disease surveillance, early detection, and the development of integrated management strategies for fusarium wilt in banana production systems.
References
Aguayo, J., Mostert, D., Fourrier-Jeandel, C., Cerf-Wendling, I., Hostachy, B., Viljoen, A., & Ioos, R. (2017). Development of a hydrolysis probe-based real-time assay for the detection of tropical strains of Fusarium oxysporum f. sp. cubense race 4. PLOS ONE, 12(2), e0171767. https://doi.org/10.1371/journal.pone.0171767
Aldayanti, R., & Ariyanti, E. L. (2023). Diversity of Rhizosphere Fungi That Have the Potential to Control the Cause of Blood Disease (Pseudomonas celebensis) in Plants. Journal Agroecotech Indonesia, 2(02), 167–178.
Baruah, A., Bora, P., Damodaran, T., Saikia, B., Manoharan, M., Patil, P., Bhattacharyya, A., Saikia, A., Kumar, A., Kumari, S., Talukdar, J., Dey, U., Ahmed, S. S., Rahman, N., Nath,
B. C., Tabing, R., & Kumar, S. (2025). Patho-Ecological Distribution and Genetic Diversity of Fusarium oxysporum f. sp. cubense in Malbhog Banana Belts of Assam, India. Journal of Fungi, 11(3), 195. https://doi.org/10.3390/jof11030195
BPS. (2024). Annual fruit and vegetable crop production by crop type. https://www.bps.go.id/id/statistics-table/3/U0dKc1owczVSalJ5VFdOMWVETnlVRVJ 6YlRJMFp6MDkjMw==/produksi-tanaman-buah-buahan-dan-sayuran-tahunan-menurut-provinsi-dan-jenis-tanaman--2024.html?year=2024
Chen, J., Cai, R., Tang, L., Wang, D., Lv, R., & Guo, C. (2024). Antagonistic activity and mechanism of Bacillus subtilis CG-6 suppression of root rot and growth promotion in Alfalfa. Microbial Pathogenesis, 190. https://doi.org/10.1016/j.micpath.2024.106616
Czislowski, E., Fraser-Smith, S., Zander, M., & Aitken, E. A. B. (2016). Identifying pathogenicity genes in Fusarium oxysporum f. sp. cubense. Acta Horticulturae, (1114), 101–106. https://doi.org/10.17660/ActaHortic.2016.1114.14
Dita, M., Barquero, M., Heck, D., Mizubuti, E. S. G., & Staver, C. P. (2018). Fusarium wilt of banana: Current knowledge on epidemiology and research needs toward sustainable disease management. In Frontiers in Plant Science (Vol. 871). Frontiers Media S.A. https://doi.org/10.3389/fpls.2018.01468
Fang, Y., & Ramasamy, R. (2015). Current and Prospective Methods for Plant Disease Detection. Biosensors, 5(3), 537–561. https://doi.org/10.3390/bios5030537
Fatma Azizah, D., & Advinda, L. (2025). Literature Review: Fusarium Wilt Disease in Banana Plants and its Control Strategies. JURNAL BIOSENSE, 8(2), 204–216. https://doi.org/10.36526/biosense.v8i2.5197
Garnica, S., Schön, M. E., Abarenkov, K., Riess, K., Liimatainen, K., Niskanen, T., Dima, B., Soop, K., Frøslev, T. G., Jeppesen, T. S., Peintner, U., Kuhnert-Finkernagel, R., Brandrud,
T. E., Saar, G., Oertel, B., & Ammirati, J. F. (2016). Determining threshold values for barcoding fungi: lessons from Cortinarius (Basidiomycota), a highly diverse and widespread ectomycorrhizal genus. FEMS Microbiology Ecology, 92(4), fiw045. https://doi.org/10.1093/femsec/fiw045
Ghag, S. B., Shekhawat, U. K. S., & Ganapathi, T. R. (2015). Fusarium wilt of banana: biology, epidemiology and management. International Journal of Pest Management, 61(3), 250–263. https://doi.org/10.1080/09670874.2015.1043972
Guo, L., Yang, L., Liang, C., Wang, G., Dai, Q., & Huang, J. (2015). Differential colonization patterns of bananas (Musa spp.) by physiological race 1 and race 4 isolates of Fusarium oxysporum f. sp. cubense. Journal of Phytopathology, 163(10), 807–817. https://doi.org/10.1111/jph.12378
Hermanto, C., Sutanto, A., Jumjunidang, Edison, H. S., Daniells, J. W., O’Neill, W. T., Sinohin, V. G. O., Molina, A. B., & Taylor, P. (2011). Incidence and Distribution of Fusarium Wilt Disease of Banana in Indonesia. Acta Horticulturae, (897), 313–322. https://doi.org/10.17660/ActaHortic.2011.897.43
Hung, T.N., Hung, N.Q., Mostert, D., Viljoen, A., Chao, C.P., Molina, A.B., 2018. First report of Fusarium wilt on Cavendish bananas, caused by Fusarium oxysporum f. sp. cubense Tropical Race 4 (VCG 01213/16), in Vietnam. Plant Disease 102 (2), 448. https://doi.org/10.1094/PDIS-08-17-1140-PDN
Isbatullah, M., Sukmawaty, E., & A. Latif, U. T. (2023). Identification of Disease Symptoms and Pathogenic Fungi on Kepok Banana Leaves (Musa acuminata x Musa balbisiana) in Samata Village, Gowa Regency, South Sulawesi. Teknosains: Media Informasi Sains Dan Teknologi, 17(1), 1–10. https://doi.org/10.24252/teknosains.v17i1.31880
Kristensen, S. B., Pedersen, T. B., Nielsen, M. R., Wimmer, R., Muff, J., & Sørensen, J. L. (2021). Production and Selectivity of Key Fusarubins from Fusarium solani due to Media Composition. Toxins, 13(6), 376. https://doi.org/10.3390/toxins13060376
Kumar, S., Stecher, G., Suleski, M., Sanderford, M., Sharma, S., & Tamura, K. (2024). MEGA12: Molecular Evolutionary Genetic Analysis Version 12 for Adaptive and Green Computing. Molecular Biology and Evolution, 41(12). https://doi.org/10.1093/molbev/msae263
Latifah, N., & Soedijo, S. (2023). Exploration and Identification of Fungi Causing Pakcoy Plant Diseases (Brassica rapa L.). 6(1).
Le Thi, L., Mertens, A., Vu, D.T., Vu, T.D., Minh, P.L.A., Duc, H.N., de Backer, S., Swennen, R., Vandelook, F., Panis, B., Amalfi, M., Decock, C., Gomes, S.I.F., Merckx, V.S.F.T., Janssens, S.B., 2022. Diversity of Fusarium associated banana wilt in northern Vietnam. MycoKeys 87, 53–76. https://doi.org/10.3897/mycokeys.87.72941
Magdama, F., Monserrate-Maggi, L., Serrano, L., García Onofre, J., & Jiménez-Gasco, M. del M. (2020). Genetic Diversity of Fusarium oxysporum f. sp. cubense, the Fusarium Wilt Pathogen of Banana, in Ecuador. Plants, 9(9), 1133.
https://doi.org/10.3390/plants9091133
Maryani, N., Lombard, L., Poerba, Y. S., Subandiyah, S., Crous, P. W., & Kema, G. H. J. (2019a). Phylogeny and genetic diversity of the banana Fusarium wilt pathogen Fusarium oxysporum f. sp. cubense in the Indonesian centre of origin. Studies in Mycology, 92, 155–194. https://doi.org/10.1016/j.simyco.2018.06.003
Maryani, N., Sandoval-Denis, M., Lombard, L., Crous, P. W., & Kema, G. H. J. (2019b). New endemic Fusarium species hitch-hiking with pathogenic Fusarium strains causing Panama disease in small-holder banana plots in Indonesia. Persoonia: Molecular Phylogeny and Evolution of Fungi, 43, 48–69. https://doi.org/10.3767/persoonia.2019.43.02
Nozawa, S., Pangilinan, D. C. J., Dionisio, G. A., & Watanabe, K. (2024). Specific PCR primer designed from genome data for rapid detection of Fusarium oxysporum f. sp. cubense tropical race 4 in the Cavendish banana. PLOS ONE, 19(12), e0313358. https://doi.org/10.1371/journal.pone.0313358
Pham, H. H., Tran, D. T., Nguyen, T. T., Thi Thanh Nguyen, M., Pham, D. T., Duong, H. Van, & Nguyen, C. X. (2024). Molecular identification of Fusarium oxysporum f. sp. cubense (Foc) causing Fusarium wilt disease of Tieu hong banana cultivar in the Red River Delta of Vietnam. Archives of Phytopathology and Plant Protection, 57(6), 470–490. https://doi.org/10.1080/03235408.2024.2366547
Ploetz, R. C. (2015). Management of Fusarium wilt of banana: A review with special reference to tropical race 4. Crop Protection, 73, 7–15. https://doi.org/10.1016/j.cropro.2015.01.007
Ramlan, R. R., Harnelly, & Fitri, L. (2024). DNA Extraction and PCR Optimization of Coffea arabica L. and Coffea canephora Pierre ex A. Froehner. Jurnal Penelitian Pendidikan
IPA, 10(SpecialIssue), 53–58. https://doi.org/10.29303/jppipa.v10iSpecialIssue.7881
Stover, N. A., & Cavalcanti, A. R. O. (2017). Using NCBI BLAST. Current Protocols Essential Laboratory Techniques, 14(1). https://doi.org/10.1002/cpet.8
Syah, M. A., Yaddi, Y., & Ulfa, N. I. (2024). Molecular Identification of Lipolytic Bacteria Isolated from Mangrove Sediments of Kendari Bay. Journal of Biological Research, 11(1), 68–77.
UPT PTPH. (2018). Report on the Status of Fruit Pest and Disease Attacks in East Java.
White, T. J., Bruns, T., Lee, S., & Taylor, J. (1990). Amplification and direct sequencing of fungal ribosomal RNA genes for phylogenetics. In PCR Protocols (pp. 315–322). Elsevier. https://doi.org/10.1016/B978-0-12-372180-8.50042-1
Zhang, L., Zhang, H., Huang, Y., Peng, J., Xie, J., & Wang, W. (2021). Isolation and Evaluation of Rhizosphere Actinomycetes with Potential Application for Biocontrolling Fusarium Wilt of Banana Caused by Fusarium oxysporum f. sp. cubense Tropical Race 4. Frontiers in Microbiology, 12. https://doi.org/10.3389/fmicb.2021.763038
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