Induction of Twisting Disease Resistance on Shallot (Allium cepa var. ascalonicum) Against Twisting Disease (Fusarium oxysporum f. sp. cepae) through Biopesticide Application

Sri Wiyatiningsih, Wahyu Santoso, Riko Setya Wijaya, Fitri Wijayanti

Abstract

This study aimed to develop resistance in shallot (Allium cepa) against twisting disease caused by Fusarium oxysporum by applying microorganism-based biopesticides. By inducing resistance in shallots, the research sought to explore the pathogen-host interactions, understand infection mechanisms, and establish sustainable disease management strategies. Utilizing a biopesticide approach within an organic farming framework, this study aimed to assess the effectiveness of biopesticides in inducing plant defense mechanisms. The experimental design was non-factorial and followed a randomized block structure, consisting of four treatments with three replications each: (A) Chemical pesticide and fertilizer, (B) Biopesticide applied to seeds and plants, with chemical fertilizers on soil, (C) Soil sterilization with biopesticide and pesticide application on plants, and (D) Soil sterilization with biopesticide, with biopesticide applied to seeds and plants. Observations focused on disease intensity and salicylic and jasmonic acid levels in shallot bulbs. Results demonstrated that biopesticide application successfully reduced disease incidence and increased resistance, as shown by higher levels of salicylic and jasmonic acids in treated plants. The study concluded that biopesticides are promising for enhancing systemic resistance in shallots.

Keywords

biocontrol; induced resistance; jasmonic acid; organic farming; salicylic acid

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References

Abbaspour, J., & Ehsanpour, A. (2016). The impact of salicylic acid on some physiological responses of Artemisia aucheri Boiss. under in vitro drought stress. Acta Agriculturae Slovenica, 107(2), 287–298. https://doi.org/10.14720/aas.2016.107.2.03

Abdelrahman, M., Ariyanti, N. A., Sawada, Y., Tsuji, F., Hirata, S., Hang, T. T. M., Okamoto, M., Yamada, Y., Tsugawa, H., Hirai, M. Y., & Shigyo, M. (2020). Metabolome-based discrimination analysis of shallot landraces and bulb onion cultivars associated with differences in the amino acid and flavonoid profiles. Molecules. https://doi.org/10.3390/molecules25225300

Akram, S., Ahmed, A., He, P., He, P., Liu, Y., Wu, Y., Munir, S., & He, Y. (2023). Uniting the Role of Endophytic Fungi against Plant Pathogens and Their Interaction. In Journal of Fungi. https://doi.org/10.3390/jof9010072

Assey, G. E., Mgohamwende, R., & Malasi, W. S. (2021). A Review of the Impact of Pesticides Pollution on Environment Including Effects , Benefits and Control. N o i t E f u f l l e o c t P s f o & l a C n o r n u t r o o J l ISSN: 2375-4397 Journal of Pollution Effects & Control, 9(4), 282. https://doi.org/10.35248/2375-4397.21.9.282

Badrudin, U., Ghulamahdi, M., Purwoko, B. S., & Pratiwi, E. (2023). Pengaruh Aplikasi Mikroba terhadap Fisiologis Beberapa Varietas Padi Fase Vegetatif pada Kondisi Salin Tergenang. Agro Bali : Agricultural Journal 6(3): 621-635. https://doi.org/10.37637/ab.v6i3.1279

Calvo-Garrido, C., Roudet, J., Aveline, N., Davidou, L., Dupin, S., & Fermaud, M. (2019). Microbial antagonism toward botrytis bunch rot of grapes in multiple field tests using one bacillus ginsengihumi strain and formulated biological control products. Frontiers in Plant Science. https://doi.org/10.3389/fpls.2019.00105

Chrapačienė, S., Rasiukevičiūtė, N., & Valiuškaitė, A. (2022). Control of Seed-Borne Fungi by Selected Essential Oils. Horticulturae. https://doi.org/10.3390/horticulturae8030220

Dean, R., Kan, J. A. N. A. L. V. A. N., Pretorius, Z. A., Hammond-kosack, K. I. M. E., Pietro, A. D. I., Spanu, P. D., Rudd, J. J., Dickman, M., Kahmann, R., Ellis, J., & Foster, G. D. (2012). The Top 10 fungal pathogens in molecular plant pathology. 13, 414–430. https://doi.org/10.1111/J.1364-3703.2011.00783.X

Elvira, N. D., Wiyatiningsih, S., & Suryaminarsih, P. (2023). Effect of Fobio on Intensity of Moler Disease (Fusarium oxysporum) on Various Shallot Cultivars. CROPSAVER - Journal of Plant Protection. https://doi.org/10.24198/cropsaver.v6i2.45747

Fadhilah, S; Wiyono; Surahman, M. (2014). Pengembangan Teknik Deteksi Fusarium Patogen Pada Umbi Benih Bawang Merah ( Allium ascalonicum ) di Laboratorium [ Development of Detection Technique for Fusarium Pathogen on Seedling Shallot ( Allium ascalonicum ) Bulb at Laboratorium ]. 24(2), 171–178.

Fauzan, M. (2020). Pendapatan Rumah Tangga Petani Bawang Merah Lahan Pasir Pantai Di Kabupaten Bantul. JAS (Jurnal Agri Sains). https://doi.org/10.36355/jas.v4i1.362

Fitriana, I. N., Suryaminarsih, P., Mindari, W., & Wiyatiningsih, S. (2020). Studi Pertumbuhan Multiantagonis Trichoderma Sp. Dan Streptomyces Sp. Dalam Suspensi Akar, Humat Cair Dan Ekstrak Kentang Gula. Berkala Ilmiah Agroteknologi - Plumula, 7(1), 25–32. https://doi.org/10.33005/plumula.v7i1.19

Gordon, T. R. (2017). Fusarium oxysporum and the Fusarium Wilt Syndrome.

Grady, E. N., MacDonald, J., Ho, M. T., Weselowski, B., McDowell, T., Solomon, O., Renaud, J., & Yuan, Z. C. (2019). Characterization and complete genome analysis of the surfactin-producing, plant-protecting bacterium Bacillus velezensis 9D-6. BMC Microbiology. https://doi.org/10.1186/s12866-018-1380-8

Gravot, S. L. A. R.-S. C. L. J. L. N. M. M. J. M. J. M.-D. A. (2015). Plant and Cell Physiology Advance Access published September 11, 2015.

Gwinn, K. D. (2018). Bioactive Natural Products in Plant Disease Control. In Studies in Natural Products Chemistry (1st ed., Vol. 56). Elsevier B.V. https://doi.org/10.1016/B978-0-444-64058-1.00007-8

Hasyidan, G. S. W. P. S. (2021). Aplikasi Biopestisida Fobio dan Streptomyces sp . Untuk Mengendalikan Penyakit Moler pada Tanaman Bawang Merah Application of Fobio Biopesticides and Streptomyces sp . to Control Moler ’ s Disease. 6(2), 168–173.

Herlina, L., Istiaji, B., & Wiyono, S. (2021). The Causal Agent of Fusarium Disease Infested Shallots in Java Islands of Indonesia. 03003, 1–10.

Jiménez-díaz, R. M., & Jiménez-gasco, M. M. (2011). Integrated Management of Fusarium Wilt Diseases. In Control of Fusarium Diseases (Vol. 661, Issue 2).

Kalman, B., Abraham, D., Graph, S., Perl-Treves, R., Harel, Y. M., & Degani, O. (2020). Isolation and identification of fusarium spp., the causal agents of onion (Allium cepa) basal rot in northeastern Israel. Biology. https://doi.org/10.3390/biology9040069

Kanno, H., Hasegawa, M., & Kodama, O. (2012). Accumulation of salicylic acid, jasmonic acid and phytoalexins in rice, Oryza sativa, infested by the white-backed planthopper, Sogatella furcifera (Hemiptera: Delphacidae). Applied Entomology and Zoology, 47(1), 27–34. https://doi.org/10.1007/s13355-011-0085-3

Kawung, Y., Tooy, D., & Pakasi, S. (2024). Design of A Web-Based Geographic Information to Show Spatial Information of Land Used for Horticulture. Agro Bali : Agricultural Journal. https://doi.org/10.37637/ab.v6i3.1373

Klessig, D. F., Choi, H. W., Dempsey, D. M. A., & Rd, T. (2018). Systemic Acquired Resistance and Salicylic Acid : Past , Present , and Future. 31(9), 871–888. https://doi.org/10.1094/MPMI-03-18-0067-CR

Maharijaya, A., Kurnianingtyas, D., Sobir, Wiyono, S., & Purwito, A. (2023). Possible Morphological And Chemical Resistance Mechanism Of Shallots (Allium Cepa Var Ascalonicum) To Colletotrichum Gloeosporioides Penz. Sabrao Journal of Breeding and Genetics. https://doi.org/10.54910/sabrao2023.55.2.26

Mihiretu, A., Assefa, N., & Wubet, A. (2024). Bridging Sorghum Yield Gap Through Up-Scaling Improved Technology in Wag-Himira Zone, Ethiopia. Agro Bali : Agricultural Journal. https://doi.org/10.37637/ab.v6i3.1117

Mullins, A. J., Murray, J. A. H., Bull, M. J., Jenner, M., Jones, C., Webster, G., Green, A. E., Neill, D. R., Connor, T. R., Parkhill, J., Challis, G. L., & Mahenthiralingam, E. (2019). Genome mining identifies cepacin as a plant-protective metabolite of the biopesticidal bacterium Burkholderia ambifaria. Nature Microbiology. https://doi.org/10.1038/s41564-019-0383-z

Ojha, Suprakash; Narayan, Chandra, C. (2012). Induction Of Resistance In Tomato Plants Against Through Salicylic Acid And Trichoderma Harzianum. Journal of Plant Protection Research, 52(2).

Omoba, O. S., Olagunju, A. I., Akinrinlola, F. O., & Oluwajuyitan, T. D. (2022). Shallot-enriched amaranth-based extruded snack influences blood glucose levels, hematological parameters, and carbohydrate degrading enzymes in streptozotocin-induced diabetic rats. Journal of Food Biochemistry. https://doi.org/10.1111/jfbc.14098

Purba, J.H., Wahyuni, P.S., Zulkarnaen, Sasmita, N., Yuniti, I G.A.D., Pandawani, N.P. 2020. Growth and yield response of shallot (Allium ascalonicum L. var. Tuktuk) from different source materials applied with liquid biofertilizers. Nusantara Bioscience 12(2):127-133. https://doi.org/10.13057/nusbiosci/n120207.

Rampersad, S. N. (2020). Pathogenomics and Management of Fusarium Diseases in Plants. i.

Safitri, Y. A., & Hasanah, U. (2019). Distribution of major diseases of shallot in South Kalimantan , Indonesia. 3(2), 33–40. https://doi.org/10.13057/asianjagric/g030201

Sullivan, C. F., Parker, B. L., & Skinner, M. (2022). A Review of Commercial Metarhizium-and Beauveria-Based Biopesticides for the Biological Control of Ticks in the USA. In Insects. https://doi.org/10.3390/insects13030260

Supyani, Hadiwiyono, Poromarto, S. H., Spriyadi, & Permatasari, F. I. (2023). Negative impact of some fungicide applications on Trichoderma harzianum as biocontrol agent of shallot moler disease. IOP Conference Series: Earth and Environmental Science. https://doi.org/10.1088/1755-1315/1180/1/012032

Tamaoki, D., Seo, S., Yamada, S., Kano, A., Miyamoto, A., Shishido, H., & Miyoshi, S. (2013). Jasmonic acid and salicylic acid activate a common defense system in rice. Plant Signaling & Behavior, May 2015, 1–4. https://doi.org/10.4161/psb.24260

Wibowo, E. P., Widiastuti, A., Joko, T., Suryanti, S., & Priyatmojo, A. (2022). Effect of Biocontrol Agent (Bacillus and Mycorrhizal Fungi) Application against Twisted Disease (Fusarium spp.) in Off-Season Shallot Production. Jurnal Perlindungan Tanaman Indonesia. https://doi.org/10.22146/jpti.75579

Wijoyo, R. B., Sulistyaningsih, E., & Wibowo, A. (2020). Growth , Yield and Resistance Responses of Three Cultivars on True Seed Shallots to Twisted Disease with Salicylic Acid Application. 35(1), 1–11.

Wiyatiningsi, S., & Sukaryorini, P. (2010). Wiyatiningsi, Sri; Sukaryorini, Pancadewi. 1, 75–80.

Xia, R., Schaafsma, A. W., Wu, F., & Hooker, D. C. (2020). Impact of the improvements in Fusarium head blight and agronomic management on economics of winter wheat. World Mycotoxin Journal, 13(3), 423–440. https://doi.org/10.3920/WMJ2019.2518

Yastika, P. E., Vipriyanti, N. U., Partama, I. G. Y., Suparwata, I. W. E., & Sudiarta, I. K. (2023). Analisis Respon Petani Terhadap Perubahan Iklim dan Curah Hujan di Subak Jatiluwih, Tabanan Bali, Indonesia. Agro Bali : Agricultural Journal. https://doi.org/10.37637/ab.v6i3.1262

DOI: https://doi.org/10.37637/ab.v7i3.1920

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