Optimizing Biotogrow Liquid Fertilizer Concentration and Application Frequency for Lettuce Cultivation Under Tropical Cambisol Conditions
Abstract
This greenhouse study investigated the impact of Biotogrow liquid organic fertilizer concentration and application frequency on lettuce (Lactuca sativa L.) growth and yield at Telaga Kodok from May to August 2024. The purpose of the research is to determine the effect of Biotogrow Liquid Fertiliser concentration and fertilisation interval, as well as the interaction between the two, on the growth and production of lettuce (Lactuca sativa L.). Using a factorial Randomized Complete Block Design with three replications, researchers tested four Biotogrow concentrations (0, 2, 5, and 8 ml L⁻¹) and four fertilization intervals (0, 4, 7, and 10 days). Growth parameters, including plant height, leaf number, leaf area, fresh weights, shoot-to-root ratio, and harvest index, were measured at 14, 21, 28, and 35 days after planting. ANOVA and Duncan's Multiple Range Test (α = 0.05) revealed significant findings. Biotogrow concentration substantially influenced plant height at 21, 28, and 35 DAP (P < 0.01), leaf number throughout all observation periods (P < 0.05 at 14 DAP; P < 0.01 thereafter), plus shoot and root fresh weight, leaf area, and harvest index (P < 0.01). Fertilization intervals significantly affected all parameters except the shoot-to-root ratio (P < 0.01). Importantly, concentration-interval interactions showed highly significant effects on leaf number at 35 DAP, shoot fresh weight, and leaf area (P < 0.01), with significant impacts on plant height at 28 and 35 DAP, leaf number at 28 DAP, and root fresh weight (P < 0.05). These results demonstrate that optimizing both Biotogrow concentration and application timing can significantly enhance lettuce productivity under greenhouse conditions.
Keywords
Full Text:
PDFReferences
Abd–Elrahman, S. H., Saudy, H. S., El–Fattah, D. A. A. & Hashem, F. A. (2022). Effect of irrigation water and organic fertilizer on reducing nitrate accumulation and boosting lettuce productivity. Journal of Soil Science and Plant Nutrition, 22(2), 2144–2155. (https://doi.org/10.1007/s42729-022-00799-8)
Abdullah, A. & Andres, J. (2021). Pengaruh pemberian pupuk organik cair terhadap pertumbuhan tanaman selada (Lactuca sativa L) secara hidroponik. Jurnal Pendas (Pendidikan Sekolah Dasar), 3(1), 21–27. https://jurnal.isdikkieraha.ac.id/index.php/pendas/article/view/189)
Anwary, M. N., Slamet, W. & Kusmiyati, F. (2019). Pertumbuhan selada merah (Lactuca sativa L. var. Red Rapid) dan selada hijau (Lactuca sativa L. Grand Rapids) dengan sistem hidroponik apung dengan pemberian dosis pupuk organik cair (POC) bioslurry dan AB mix yang berbeda. Buletin Anatomi Dan Fisiologi, 4(2), 160–167. (https://doi.org/10.14710/baf.4.2.2019.160-167)
Carong, N. F. R. & Rafiuddin, M. K. (2025). Arbuscular mycorrhiza fungi and mineral fertilizer effects on growth and production traits of the potato (Solanum tuberosum L.). SABRAO Journal of Breeding & Genetics, 57(2). (http://doi.org/10.54910/sabrao2025.57.2.31.)
Cintiyah, F., Salundik, S. & Komala, I. (2024). Optimization of liquid organic fertilizer from livestock manure with Indigofera for hydroponic lettuce growth. Agro Bali: Agricultural Journal, 7(3), 676–690. (https://doi.org/10.37637/ab.v7i3.1875)
Dewi Ratna Nurhayati, W. A. A. (2023). Effect of Biotogrow Liquid Fertilizer Dosage and Interval on Green Eggplant (Solanum Melongena L.) Growth and Yield. Nongye Jixie Xuebao/Transactions of the Chinese Society of Agricultural Machinery, 54(4). (https://nyjxxb.net/index.php/journal/article/view/1587/1581)
Ekinci, M., Yıldırım, E., Turan, M. & Kul, R. (2020). Organic Fertilizers Improved Plant Growth and Mineral Content of Lettuce (Lactuca sativa L.). Erciyes Tarım ve Hayvan Bilimleri Dergisi, 3(2), 1–5. (https://dergipark.org.tr/en/download/article-file/1282265)
Frasetya, B., Harisman, K. & Ramdaniah, N. A. H. (2021). The effect of hydroponics systems on the growth of lettuce. IOP Conference Series: Materials Science and Engineering, 1098(4), 042115. (DOI 10.1088/1757-899X/1098/4/042115)
Hamawi, M., Akhiriana, E. & Marwatun, S. (2024). Pengaruh Pupuk Organik Cair (POC) Bekatul Terhadap Pertumbuhan Selada (Lactuca sativa L.) yang dibudidayakan Secara Hidroponik. Agroteknika, 7(2), 275–286. (https://doi.org/10.55043/agroteknika.v7i2.200)
Hasan, M. M. & Jho, E. H. (2023). Effect of different types and shapes of microplastics on the growth of lettuce. Chemosphere, 339, 139660. (https://doi.org/10.1016/j.chemosphere.2023.139660)
Hasniar, H., Iinnaninengseh, I. & Satriani, S. (2022). Pengaruh Media Tanam Yang Berbeda Dan Pemberian Dosis Pupuk Organik Cair Nasa Terhadap Pertumbuhan Dan Hasil Tanaman Selada (Lactuca Sativa L.). Jurnal Agroterpadu, 1(1), 13–17. (http://dx.doi.org/10.35329/ja.v1i1.2815)
He, X., Zhu, H., Shi, A. & Wang, X. (2024). Optimizing nitrogen fertilizer management enhances rice yield, dry matter, and nitrogen use efficiency. Agronomy, 14(5), 919. (https://doi.org/10.3390/agronomy14050919)
Ilmi, A. A., Santoso, J. & Sutini, S. (2025). The Effect of Bio-Fertilizer Concentration and NPK Fertilizer Dosage on the Growth and Yield of Melon (Cucumis melo L.) Merlin Variety. Jurnal Teknik Pertanian Lampung (Journal of Agricultural Engineering), 14(2), 638–644. (https://doi.org/10.23960/jtep-l.v14i2.638-644)
Januarisky, H. A., Syaukat, Y. & Rifin, A. (2025). The Effect of Urea and NPK Fertilizer Usage on Farmers Receiving Fertilizer Subsidies. Agro Bali: Agricultural Journal, 8(2), 352–363. (https://doi.org/10.37637/ab.v8i2.2098)
Legendre, R. & van Iersel, M. W. (2021). Supplemental far-red light stimulates lettuce growth: Disentangling morphological and physiological effects. Plants, 10(1), 166. (https://doi.org/10.3390/plants10010166)
Li, J., Liu, Y., Tang, Y., Shao, J., Xu, T., Ma, R., Jiang, Y. & Cheng, D. (2022). Optimizing fertilizer management based on controlled-release fertilizer to improve yield, quality, and reduce fertilizer application on apples. Journal of Soil Science and Plant Nutrition, 22(1), 393–405. (https://doi.org/10.1007/s42729-021-00656-0)
Muscolo, A., Marra, F., Canino, F., Maffia, A., Mallamaci, C. & Russo, M. (2022). Growth, nutritional quality and antioxidant capacity of lettuce grown on two different soils with sulphur-based fertilizer, organic and chemical fertilizers. Scientia Horticulturae, 305, 111421. (https://doi.org/10.1016/j.scienta.2022.111421)
Nurhayati, D. R. (2023). Effect of Biotogrow Dosage on Black and White Sesame Varieties Quality. Journal of Social Science, 4(1), 1–15. (https://doi.org/10.46799/jss.v4i1.499)
Pawase, P. P., Nalawade, S. M., Bhanage, G. B., Walunj, A. A., Kadam, P. B., Durgude, A. G. & Patil, M. R. (2023). Variable rate fertilizer application technology for nutrient management: A review. International Journal of Agricultural and Biological Engineering, 16(4), 11–19. (10.25165/j.ijabe.20231604.7671)
Putri, O. S. & Rahmayuni, E. (2023a). Aplikasi Pupuk Organik Cair Daun Lamtoro terhadap Pertumbuhan dan Produksi Selada Merah. Jurnal Hortikultura Indonesia (JHI), 14(3), 177–183. (https://doi.org/10.29244/jhi.14.3.177-183)
Putri, O. S. & Rahmayuni, E. (2023b). Aplikasi Pupuk Organik Cair Daun Lamtoro terhadap Pertumbuhan dan Produksi Selada Merah. Jurnal Hortikultura Indonesia (JHI), 14(3), 177–183. (https://doi.org/10.29244/jhi.14.3.177-183)
Qiao, L., Wang, X., Smith, P., Fan, J., Lu, Y., Emmett, B., Li, R., Dorling, S., Chen, H. & Liu, S. (2022). Soil quality both increases crop production and improves resilience to climate change. Nature Climate Change, 12(6), 574–580. (https://doi.org/10.1038/s41558-022-01376-8)
Rai, I. N., Wijana, G. & Mintarajasa, J. D. (2025). Effect of Fertilization Package on Vegetative Growth of Tejakula Tangerine (Citrus reticulata cv. Tejakula) After Transplanting to the Field. Agro Bali: Agricultural Journal, 8(1), 137–144. (https://doi.org/10.37637/ab.v)
Ramadhan, R., Syah, B. & Sugiono, D. (2021). Pengaruh kombinasi dosis pupuk organik cair dan pupuk NPK majemuk terhadap pertumbuhan dan hasil tanaman selada keriting (Lactuca sativa L.) varietas Grand Rapids pada sistem vertikultur. Jurnal Ilmiah Wahana Pendidikan, 7(5), 106–117. (https://doi.org/10.5281/zenodo.5502836)
Ria, P., Noer, S. & Marhento, G. (2021). Efektivitas Pemberian Nasi Basi Sebagai Pupuk Organik pada Tanaman Selada Merah (Lactuca sativa var. crispa). EduBiologia: Biological Science and Education Journal, 1(1), 55–61. (http://dx.doi.org/10.30998/edubiologia.v1i1.8088)
Shaik, A., Singh, H., Singh, S., Montague, T. & Sanchez, J. (2022). Liquid organic fertilizer effects on growth and biomass of lettuce grown in a soilless production system. HortScience, 57(3), 447–452. (10.21273/HORTSCI16334-21)
Shatilov, M. V, Razin, A. F. & Ivanova, M. I. (2019). Analysis of the world lettuce market. IOP Conf Ser Earth Environ Sci. 395: 012053. (10.1088/1755-1315/395/1/012053)
Sulaeman, Y., Cahyana, D. & Nursyamsi, D. (2021). Spatial Identification of Black Soils in Indonesia. IOP Conference Series: Earth and Environmental Science, 757(1), 012035. (10.1088/1755-1315/757/1/012035)
Thomas, T., Biradar, M. S., Chimmad, V. P. & Janagoudar, B. S. (2021). Growth and physiology of lettuce (Lactuca sativa L.) cultivars under different growing systems. Plant Physiology Reports, 26(3), 526–534. (https://doi.org/10.1007/s40502-021-00591-3)
Zhou, Z., Wang, C. & Luo, Y. (2020). Meta-analysis of the impacts of global change factors on soil microbial diversity and functionality. Nature Communications, 11(1), 3072. (https://doi.org/10.1038/s41467-020-16881-7)
Refbacks
- There are currently no refbacks.

























